Part:BBa_K5140001
Copper ion-induced absolute bacterial kill switch
The highly efficient T7 promoter was used to prevent the unintended induction of cell death. CueR (https://parts.igem.org/Part:BBa_K1758324), synthesized by this promoter, binds to Cu+ reduced by the cell, activating the copA promoter, which synthesizes TetR (https://parts.igem.org/Part:BBa_K1475003). TetR then binds to tetO, suppressing the tet promoter (https://parts.igem.org/Part:BBa_R0040) and preventing the production of biotoxins. However, outside of the medium, Cu+ cannot bind, and the copA promoter is inhibited. As a result, the tet promoter becomes active, and Holin (https://parts.igem.org/Part:BBa_K1378031) forms holes in the bacterial inner membrane. Endolysin (https://parts.igem.org/Part:BBa_K1378032) then passes through the holes created in the inner membrane, destroying the cell wall and leading to the death of the organism.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 44
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 55
Illegal NheI site found at 78
Illegal SpeI site found at 44 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 44
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 44
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 523
Illegal SapI.rc site found at 673
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